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BETTER WHEY TO GROW ON A KETO DIET: AML™ THERMO HEAT® FAT BURNING PROTEIN!

Brian Turner

Posted on May 10 2019

By Steve Blechman

 

Research has shown that taking 6.25g of whey protein with a high dose of leucine (5g) can enhance protein synthesis at the same rate as four times as much whey protein (25g)! Leucine is the key anabolic trigger of muscle protein synthesis. Increases in muscle protein synthesis are dependent on leucine concentration. 5 grams of leucine can increase the anabolic effects of protein synthesis when eating less protein, which is ideal on a ketogenic diet. Older adults (people over 40) show a blunted muscle protein synthesis (MPS) and anabolic resistance compared to younger adults. Recent research has suggested that the anabolic resistance can be overcome by consuming greater quantities of leucine. In a recent study, published in the Journal of Nutrition on July 1, 2018, it is confirmed that leucine and not total protein content of a “… supplement is the primary determinant of muscle protein anabolic responses in healthy older women.” Lower-protein supplements with added leucine may represent an advantageous approach in older adults to maintain skeletal muscle anabolic sensitivity and attenuate muscle loss.

Research has shown that pure leucine is more anabolic than leucine in food (Nutrients, October 2018). Leucine is the key amino acid for enhancing the mTOR pathway that regulates cell growth and protein synthesis. Leucine is an essential amino acid that serves as a building block for muscle protein synthesis. Leucine is a powerful anabolic trigger— it’s the most potent branched-chain amino acid (BCAA) and a key activator of the mTOR pathway that is critical for muscle protein synthesis that promotes muscle growth. Testosterone acts through the mTOR pathway to promote muscle protein synthesis. Leucine has many benefits: powering muscle growth, preventing muscle loss, increasing insulin sensitivity, enhancing fat metabolism and enhancing recovery.

A most recent study has found that taking pure leucine supplements alone (or with very small amounts of whey protein) may be better for muscle protein synthesis and more anabolic than food! In a randomized crossover study (Nutrients, October 18, 2018) 10 healthy, young Japanese men underwent tests under different conditions: consuming 2 grams of leucine alone; a mixed meal with 2.15 grams of leucine without any additional leucine supplementation; 2 grams of leucine right after a meal; and the final serving consisted of 2 grams of leucine, 180 minutes after a meal.

The study’s conclusion was, “based on the aforementioned discussions, the intake of free leucine alone markedly increased the plasma leucine concentration. However, the increase in leucine concentration after the intake of a mixed meal containing the same amount of leucine was significantly less than that of free leucine intake alone. Moreover, when free leucine was ingested after a mixed meal with the purpose of increasing the plasma leucine concentration, the maximum plasma concentration was attenuated when it was ingested immediately after the mixed meal, despite the fact that the total leucine content was doubled. These results suggest that when free amino acids are ingested with the purpose of increasing plasma amino acid concentrations, the timing in relation to the mixed meal intake needs to be considered.”

Increase in muscle protein synthesis is dependent on leucine concentration. Research has shown that leucine stimulates the anabolic effect of muscle protein synthesis on its own (Wilkinson et al., J Physiol 2013). When leucine is taken on an empty stomach (or with low amounts of whey protein), it has a powerful anabolic switch that turns on protein synthesis.

By taking pure leucine on an empty stomach (or with small amounts whey protein), you will get a better spike in blood levels than if you take leucine with food, because food can slow leucine’s absorption. The addition of isoleucine and valine may hinder the benefits of leucine due to competition for transport into muscle cells. Leucine supplements increase mTOR activity for several hours after ingestion. This makes AML™ THERMO HEAT® FAT BURNING PROTEIN more anabolic than 100% whey protein! As we said earlier, when 5 grams of leucine is taken with small amounts of whey protein (6.25 grams), it can spike blood levels of leucine and mTOR activation. The combination will also provide all the essential amino acids for protein synthesis.

AML™ THERMO HEAT® FAT BURNING PROTEIN contains potassium and magnesium citrate. These are added as important electrolytes and acid buffers (alkalinizers) when following low-carb and ketogenic diets. Low-grade chronic acidosis (which can increase muscle protein breakdown and bone loss), dehydration, electrolyte (mineral) loss and kidney stones are more prevalent in those people following a low-carb or ketogenic diets. Potassium citrate may help preserve lean body mass by reducing blood acidity in those following a low-carb, ketogenic diet. Also, recent research has shown that potassium citrate can help prevent kidney stones in those people following a low-carb or ketogenic diet!

AML™ THERMO HEAT® FAT BURNING PROTEIN is also more effective in burning fat than other proteins. Research has shown that leucine can enhance fat loss and promote weight loss during energy restriction. Leucine improves mitochondrial biogenesis and fatty acid oxidation and fat burning through SIRT-1 and AMPK-dependent pathways. AMPK (5 adenosine-monophosphate-activated protein kinase) regulates energy expenditure by modulating NAD metabolism and SIRT-1 activity. Leucine also increases glucagon-like peptide-1 (GLP-1), often referred to as the “satiety hormone.” Leucine is a solely ketogenic amino acid; it is not glucogenic (it is not converted to glucose in the liver). Isoleucine is ketogenic and glucogenic and valine is 100% glucogenic. Whey protein also contains other glucogenic amino acids that may not be desirable in those people following a ketogenic diet. By keeping the whey protein in the AML™ THERMO HEAT® FAT BURNING PROTEIN low and boosting the leucine, this will keep isoleucine and valine low— which are both glucogenic amino acids, especially valine. A most recent study found that valine has no effect on weight loss or satiety in healthy adults (Nutrients, January 5, 2019).

AML™ THERMO HEAT® FAT BURNING PROTEIN contains 5 grams of citrulline per serving, which can increase nitric oxide. Citrulline can also enhance muscle growth by stimulating the expression of IGF-1 and skeletal muscle peroxisome proliferator-activated receptor-gamma co-activator (PGC-1 alpha), which increases mitochondrial biogenesis and lean muscle mass! PGC-1 alpha can also enhance fat burning, thermogenesis, brown fat activation and mitochondria uncoupling protein 1 (UCP1). Plus, prolong exercise time to exhaustion and improve exercise performance (Molecular Nutrition Food Research, June 2018).

Citrulline also increases brown fat activity, thereby increasing caloric expenditure and fat burning. Citrulline and arginine enhance nitric oxide, brown fat activity and thermogenesis. Citrulline is a more effective supplement than arginine for promoting intracellular nitric oxide! The more brown adipose tissue (BAT), the more calories you burn. Citrulline has also been shown to reduce blood sugar production in the liver and stimulate abdominal fat loss. In addition, citrulline has also been shown to increase levels of hormone-sensitive lipase by 150%, further enhancing fat burning. Nitric oxide promotes fat oxidation and fat suppression. It also regulates feeding and satiation (Appetite, January 1, 2019, Journal of Nutrition, February 2009). A randomized double-blind placebo controlled clinical trial found that 2 grams of citrulline over 12 weeks lowered inflammation and c-reactive proteins (BMC Research, 2019).

AML™ THERMO HEAT® FAT BURNING PROTEIN also contains other nutrients that have been shown to increase nitric oxide. Nitric oxide has been shown to activate BAT and thermogenesis. Not only is citrulline supplementation good for increasing nitric oxide, but also grapeseed extract has been shown to increase nitric oxide production. Polyphenols are being studied on their role in fat metabolism and obesity management. Folic acid also boosts nitric oxide availability, by increasing BH4 and decreasing homocysteine levels. Research has shown that folic acid can lower homocysteine levels, increase insulin sensitivity and lower fasting insulin levels in type 2 diabetes. Medium-chain triglycerides (MCTs), grains of paradise (40mg - standardized for 12% paradol, a clinically effective dose) and BioPerine® black pepper fruit extract are all included for further activation of brown adipose tissue (BAT) and thermogenesis. Grains of paradise, a spice containing 6-paradol, like chili peppers containing capsaicin, activate BAT, increase whole-body energy expenditure, and decrease visceral fat (deep abdominal fat) in humans. Allulose is added as a natural, low-calorie, fat-burning, thermogenic sweetener. It is approved for low-sugar/low-carb or ketogenic diets. Allulose does not impact blood sugar or insulin levels.

Medium-chain triglycerides (MCTs) naturally increase the production of ketones in the liver and stimulate ketogenesis. Research has shown that MCT supplements do not cause feedback inhibition or reduce ketone production. MCTs also help reduce appetite and food intake, enhance fatty acid oxidation, fat burning, energy expenditure, and thermogenesis and reduce body fat accumulation in humans. Research has shown that MCTs (a saturated fat) also do not have a negative effect on blood lipids in humans. MCTs significantly increase “good” HDL cholesterol with no effect on triglycerides or “bad” LDL cholesterol. Don’t confuse MCTs with coconut oil. Claims that the majority of fat in coconut oil are MCTs is untrue. The thermogenic/weight-loss research has been done on pure MCT oil, not coconut oil. It’s inaccurate to apply the research on pure 100% MCT oil to coconut oil, which only has 13% of MCTs.

Research has shown that vitamin D-3 is the most active form of vitamin D and can potentiate protein synthesis and the muscle-enhancing effects of leucine; and is added to help boost the lean body mass effect of leucine. A randomized controlled trial found that vitamin D-3 combined with leucine and medium-chain triglycerides (MCTs) can increase muscle strength and function (Journal of Nutrition, 2016.) Also, recent research has shown that vitamin D supplements may enhance fat loss and improve waist-hip ratio and fasting blood glucose in vitamin D-deficient individuals.

AML™ THERMO HEAT® FAT BURNING PROTEIN does not contain pure ketone supplements such as beta-hydroxybutyrate (BHB). Ketone supplements in the form of BHB salts and ketone esters only provide a temporary increase in blood ketone levels. Ingesting exogenous ketones may briefly interrupt your body’s natural production of ketones. BHB salts do not stimulate ketogenesis. Ketone supplementation reduces lipolysis and free fatty acids availability and may inhibit the body’s natural ability to burn its own body fat for fuel. A most recent study has shown that feeding ketone BHB supplements in healthy humans may have an undesirable effect by activating the innate immune response and increasing inflammation. If you want to get the anti-catabolic and fat-loss effects of a ketogenic diet, ketone supplements are not desirable. Also, ketone supplements such as calcium BHB are high in calcium; people on ketogenic diets are at higher risk of kidney stones. High serum calcium levels can increase the risk of kidney stones in susceptible individuals. High calcium supplementation has also been linked to an increase risk of cardiovascular disease. Ketone supplements do not build muscle, lean body mass, or enhance weight loss, fat burning or cognitive function, according to the recent research (Applied Physiology, Nutrition and Metabolism, 2018; Frontiers in Physiology, 2017). Medium-chain triglycerides (MCT) naturally increase production of ketones in the liver and stimulate ketogenesis in humans. Research has shown that MCTs do not cause feedback inhibition or reduced ketone production. MCT stimulate the body’s natural ketone generation.

The book entitled Thermo Heat® Weight Loss Revolution by Michael Rudolph, Ph.D. outlines, in chapter 2, The Thermo Heat HIIT Workout, plus The Thermo Heat Total-Body Fat-Incinerating Exercise Plan. The book also provides a calorie-controlled, low-carb Mediterranean diet, 30-day meal plan and exercise program. It says that you should limit yourself to 100 grams of carbohydrates per day, or less. If you want to follow the ketogenic diet, carbohydrates should be kept at less than 50 grams per day.

In conclusion, just as the title states, NEW AML™ THERMO HEAT® FAT BURNING PROTEIN is the better whey to grow and is a more potent anabolic and fat burner than other protein sources! No lower-quality hydrolyzed collagen protein is present! It is designed and formulated based on the latest scientific research on preserving lean body mass and enhancing fat loss on a low-calorie and low-carb ketogenic diet and as part of an aerobic and resistance training program. Losing muscle mass during dieting can decrease one’s metabolic rate and result in burning fewer calories over a 24-hour period. As we stated earlier, 6.25g of whey protein with a high dose of leucine (5g) can enhance protein synthesis at the same rate as four times as much whey protein (25g); this is ideal for people on the ketogenic diet. Eating too much protein on the ketogenic diet can throw you out of ketosis, which isn’t desirable. Remember, a healthy weight loss is one that enhances reduction of body fat and preserves lean body mass while dieting.

 

References: 

  1. Yoshii et al. Nutrients 2018, October 18, 2018 (10), 1543; https://doi.org/10.3390/nu10101543 Effect of Mixed Meal and Leucine Intake on Plasma Amino Acid Concentrations in Young Men.
  1. Tyler A Churchward-Venne, Leigh Breen, Danielle M Di Donato, Amy J Hector, Cameron J Mitchell, Daniel R Moore, Trent Stellingwerff, Denis Breuille, Elizabeth A Offord, Steven K Baker, Stuart M Phillips, Leucine supplementation of a low-protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: a double-blind, randomized trial, The American Journal of Clinical Nutrition, Volume 99, Issue 2, February 2014, Pages 276-286, https://doi.org/10.3945/ajcn.113.068775 
  1. Rigamonti, A.E.; Leoncini, R.; Casnici, C.; Marelli, O.; De Col, A.; Tamini, S.; Lucchetti, E.; Cicolini, S.; Abbruzzese, L.; Cella, S.G.; Sartorio, A. Whey Proteins Reduce Appetite, Stimulate Anorexigenic Gastrointestinal Peptides and Improve Glucometabolic Homeostasis in Young Obese Women. Nutrients 2019, 11, 247.
  1. Chen Q, Reimer RA. Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro. Nutrition. 2008;25(3):340-9. 
  1. Devries MC, Philips SM, Baker SK et al. Leucine, Not Total Protein, Content of a Supplement Is the Primary Determinant of Muscle Protein Anabolic Responses in Healthy Older Women. The Journal of Nutrition, July 1, 2018. 
  1. Sara Y Oikawa, Chris McGlory, Lisa K D'Souza, Adrienne K Morgan, Nelson I Saddler, Steven K Baker, Gianni Parise, Stuart M Phillips, A randomized controlled trial of the impact of protein supplementation on leg lean mass and integrated muscle protein synthesis during inactivity and energy restriction in older persons, The American Journal of Clinical Nutrition, Volume 108, Issue 5, November 2018, Pages 1060-1068, https://doi.org/10.1093/ajcn/nqy193 
  1. Li H, Xu M, Lee J, He C and Xie Z. (2012). Leucine supplementation increases SIRT1 expression and prevents mitochondrial dysfunction and metabolic disorders in high-fat diet-induced obese mice. American Journal of Physiology - Endocrinology and Metabolism, 303(10), E1234-E1244. http://doi.org/10.1152/ajpendo.00198.2012 
  1. Chunzi Liang, Benjamin J Curry, Patricia L Brown and Michael B. Zemel, “Leucine Modulates Mitochondrial Biogenesis and SIRT1-AMPK Signaling in C2C12 Myotubes,” Journal of Nutrition and Metabolism, vol. 2014, Article ID 239750, 11 pages, 2014. https://doi.org/10.1155/2014/239750
  1. Wilkinson DJ et al. Effects of leucine and its metabolite beta-hydroxy-beta-methylbutyrate on human skeletal muscle protein metabolism. J Physiol 2013,591,2911-2923.
  1. Zhang Y, Guo K, LeBlanc RE, Loh D, Schwartz GJ, Yu YH. Increasing dietary leucine intake reduces diet-induced obesity and improves glucose and cholesterol metabolism in mice via multimechanisms. Diabetes 2007; 56: 164754.
  1. Binder E, Bermúdez-Silva FJ, André C, Elie M, Romero-Zerbo SY, et al. Leucine Supplementation Protects from Insulin Resistance by Regulating Adiposity Levels (2013) PLOS ONE 8(9): e74705. https://doi.org/10.1371/journal.pone.0074705
  1. Li H, Xu M, Lee J, He C and Xie Z (2012). Leucine supplementation increases SIRT1 expression and prevents mitochondrial dysfunction and metabolic disorders in high-fat diet-induced obese mice. American journal of physiology. Endocrinology and metabolism, 303(10), E1234-44. 
  1. Grape seed and skin extract mitigates heart and liver oxidative damage induced by a high-fat diet in the rat: gender dependency. Kamel Charradi, Mohamed Mahmoudi, Salem Elkahoui, Ferid Limam, Ezzedine Aouani. Canadian Journal of Physiology and Pharmacology, 2013, 91:1076-1085, https://doi.org/10.1139/cjpp-2013-0225
  1. Effects of a Vitamin D and Leucine-Enriched Whey Protein Nutritional Supplement on Measures of Sarcopenia in Older Adults, the PROVIDE Study: A Randomized, Double-Blind, Placebo-Controlled Trial. Bauer, Jürgen M. et al. Journal of the American Medical Directors Association, Volume 16, Issue 9, 740-747. September 201 
  1. C. Carpene, S. Gomez-Zorita, S. Deleruyelle and M.A. Carpene, “Novel Strategies for Preventing Diabetes and Obesity Complications with Natural Polyphenols”, Current Medicinal Chemistry (2015) 22: 150. https://doi.org/10.2174/0929867321666140815124052
  1. Grape seed and skin extract reduces pancreas lipotoxicity, oxidative stress and inflammation in high fat diet fed rats. Biomedicine & pharmacotherapy, Vol: 84, Page: 2020-2028 Aloui, F; Charradi, K; Hichami, A et al; December 2016 DOI10.1016/j.biopha.2016.11.017 
  1. Cliff J. d C. Harvey, Grant M. Schofield, Micalla Williden, and Joseph A. McQuillan, “The Effect of Medium Chain Triglycerides on Time to Nutritional Ketosis and Symptoms of Keto-Induction in Healthy Adults: A Randomised Controlled Clinical Trial,” Journal of Nutrition and Metabolism, vol. 2018, Article ID 2630565, 9 pages, 2018. https://doi.org/10.1155/2018/2630565.
  1. Nisha Panth, Kylie A Abbott, Cintia B Dias, Katie Wynne, Manohar L Garg, Differential effects of medium- and long-chain saturated fatty acids on blood lipid profile: a systematic review and meta-analysis, The American Journal of Clinical Nutrition, Volume 108, Issue 4, October 2018, Pages 675-687, https://doi.org/10.1093/ajcn/nqy167 
  1. McNally MA, Pyzik PL, Rubenstein JE, Hamdy RF, Kossoff EH. Empiric use of potassium citrate reduces kidney-stone incidence with the ketogenic diet. Pediatrics. 2009;124(2):e300-4.
  1. Sampath, A., Kossoff, E. H., Furth, S. L., Pyzik, P. L., & Vining, E. P. G. (2007). Kidney Stones and the Ketogenic Diet: Risk Factors and Prevention. Journal of Child Neurology, 22(4), 375-378. https://doi.org/10.1177/0883073807301926
  1. Dawson-Hughes B, Harris SS, Ceglia L. Alkaline diets favor lean tissue mass in older adults. Am J Clin Nutr. 2008;87(3):662-5. 
  1. Elovaris RA, Fitzgerald PCE, Bitarafan V, Ullrich SS, Horowitz M, Feinle-Bisset C. Intraduodenal Administration of L-Valine Has No Effect on Antropyloroduodenal Pressures, Plasma Cholecystokinin Concentrations or Energy Intake in Healthy, Lean Men. Nutrients. 2019;11(1):99. Published 2019 Jan 5. doi:10.3390/nu11010099 
  1. Farhat, G., Drummond, S., and Al‐Dujaili, E. A. S. (2017) Polyphenols and Their Role in Obesity Management: A Systematic Review of Randomized Clinical Trials. Phytother. Res., 31: 1005-1018. doi: 10.1002/ptr.5830.
  1. Joffin, N., Jaubert, A., Durant, S., Bastin, J., De Bandt, J., Cynober, L., Moinard, C., Coumoul, X., Forest, C. and Noirez, P. (2014), Citrulline reduces glyceroneogenesis and induces fatty acid release in visceral adipose tissue from overweight rats. Mol. Nutr. Food Res., 58: 2320-2330. doi:10.1002/mnfr.201400507
  1. Joffin, N., Jaubert, A., Durant, S., Bastin, J., De Bandt, J., Cynober, L., Moinard, C., Forest, C. and Noirez, P. (2014), Citrulline induces fatty acid release selectively in visceral adipose tissue from old rats. Mol. Nutr. Food Res., 58: 1765-1775. doi:10.1002/mnfr.201400053 
  1. Joffin N, Jaubert AM, Bamba J, Barouki R, Noirez P, Forest C. Acute induction of uncoupling protein 1 by citrulline in cultured explants of white adipose tissue from lean and high-fat-diet-fed rats. Adipocyte. 2015;4(2):129-34. Published 2015 Jan 7. doi:10.4161/21623945.2014.989748
  1. Joffin, N., Jaubert, A., Durant, S., Bastin, J., De Bandt, J., Cynober, L., Moinard, C., Coumoul, X., Forest, C. and Noirez, P. (2014), Citrulline reduces glyceroneogenesis and induces fatty acid release in visceral adipose tissue from overweight rats. Mol. Nutr. Food Res., 58: 2320-2330. doi:10.1002/mnfr.201400507 
  1. Villareal MO, Matsukawa T, Isoda H. L-Citrulline Supplementation-Increased Skeletal Muscle PGC-1α Expression is Associated With Exercise Performance and Increased Skeletal Muscle Weight [published online ahead of print, 2018 Jun 25]. Mol Nutr Food Res. 2018;62(14):e1701043. doi:10.1002/mnfr.20170104 
  1. Impact of 3-week citrulline supplementation on postprandial protein metabolism in malnourished older patients: The Ciproage randomized controlled trial (2018) Bouillanne, Olivier et al. Clinical Nutrition, Volume 38, Issue 2, 564-574 
  1. Yoshitomi H, Momoo M, Ma X, et al. L-Citrulline increases hepatic sensitivity to insulin by reducing the phosphorylation of serine 1101 in insulin receptor substrate-1. BMC Complement Altern Med. 2015;15:188. Published 2015 Jun 18. doi:10.1186/s12906-015-0706-4 
  1. Nitric oxide and l-arginine regulate feeding in satiated rats. Hazut, Noa; Rapps, Kayla; Kristt, Donald A; Susswein, Abraham J; Weller, Aron. Data Appetite, Vol: 132, Page: 44-54.2019. DOI10.1016/j.appet.2018.09.023 
  1. Jobgen W, Meininger CJ, Jobgen SC, et al. Dietary L-arginine supplementation reduces white fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats. J Nutr. 2009;139(2):230-7. 
  1. A decrease in glucose production is associated with an increase in plasma citrulline response to oral arginine in normal volunteers. Apostol, Anthony T et al. Metabolism - Clinical and Experimental, November 2003, Volume 52, Issue 11, 1512-1516 
  1. Sansbury BE, Hill BG. Regulation of obesity and insulin resistance by nitric oxide. Free Radic Biol Med. 2014;73:383-99. 
  1. Neudorf, H., Durrer, C., Myette‐Cote, E., Makins, C., O'Malley, T. and Little, J. P. (2019), Oral Ketone Supplementation Acutely Increases Markers of NLRP3 Inflammasome Activation in Human Monocytes. Mol. Nutr. Food Res. Accepted Author Manuscript. doi:10.1002/mnfr.201801171 
  1. Kevin J Acheson, Anny Blondel-Lubrano, Sylviane Oguey-Araymon, Maurice Beaumont, Shahram Emady-Azar, Corinne Ammon-Zufferey, Irina Monnard, Stéphane Pinaud, Corine Nielsen-Moennoz, Lionel Bovetto, Protein choices targeting thermogenesis and metabolism. The American Journal of Clinical Nutrition, Volume 93, Issue 3, March 2011, Pages 525-534, https://doi.org/10.3945/ajcn.110.005850 
  1. Whey protein preloads are more beneficial than soy protein preloads in regulating appetite, calorie intake, anthropometry, and body composition of overweight and obese men. Tahavorgar, Atefeh; Vafa, Mohammadreza; Shidfar, Farzad; Gohari, Mahmoodreza; Heydari, Iraj. 1879-0739, Vol: 34, Issue: 10, Page: 856-61, 2014. DOI10.1016/j.nutres.2014.08.015 
  1. Larsen AE, Bibby BM, Hansen M. Effect of a Whey Protein Supplement on Preservation of Fat Free Mass in Overweight and Obese Individuals on an Energy Restricted Very Low Caloric Diet. Nutrients. 2018;10(12):1918. Published 2018 Dec 4. doi:10.3390/nu10121918
  1. Sakiko Abe, Osamu Ezaki, Motohisa Suzuki, Medium-Chain Triglycerides in Combination with Leucine and Vitamin D Increase Muscle Strength and Function in Frail Elderly Adults in a Randomized Controlled Trial, The Journal of Nutrition, Volume 146, Issue 5, May 2016, Pages 1017-1026, https://doi.org/10.3945/jn.115.228965 
  1. Audrey Chanet, Sjors Verlaan, Jérôme Salles, Christophe Giraudet, Véronique Patrac, Véronique Pidou, Corinne Pouyet, Nordine Hafnaoui, Adeline Blot, Noël Cano, Nicolas Farigon, Anke Bongers, Marion Jourdan, Yvette Luiking, Stéphane Walrand, Yves Boirie, Supplementing Breakfast with a Vitamin D and Leucine-Enriched Whey Protein Medical Nutrition Drink Enhances Postprandial Muscle Protein Synthesis and Muscle Mass in Healthy Older Men, The Journal of Nutrition, Volume 147, Issue 12, December 2017, Pages 2262–2271, https://doi.org/10.3945/jn.117.252510
  1. Amely M Verreijen, Sjors Verlaan, Mariëlle F Engberink, Sophie Swinkels, Johan de Vogel-van den Bosch, Peter JM Weijs, A high whey protein-, leucine-, and vitamin D-enriched supplement preserves muscle mass during intentional weight loss in obese older adults: a double-blind randomized controlled trial, The American Journal of Clinical Nutrition, Volume 101, Issue 2, February 2015, Pages 279-286, https://doi.org/10.3945/ajcn.114.090290 
  1. Salehpour A, Hosseinpanah F, Shidfar F, et al. A 12-week double-blind randomized clinical trial of vitamin D₃ supplementation on body fat mass in healthy overweight and obese women. Nutr J. 2012;11:78. Published 2012 Sep 22. doi:10.1186/1475-2891-11-78
  1. Vitamin D decreases adipocyte lipid storage and increases NAD-SIRT1 pathway in 3T3-L1 adipocytes. Chang, Eugene; Kim, Yangha. Nutrition, 2016, Vol: 32, Issue: 6, Page: 702-8 10.1016/j.nut.2015.12.032 
  1. A Low-Carbohydrate Ketogenic Diet Reduces Body Mass Without Compromising Performance in Powerlifting and Olympic Weightlifting Athletes. Greene, David A.; Varley, Benjamin J.; Hartwig, Timothy B.; Chapman, Phillip; Rigney, Michael. The Journal of Strength & Conditioning Research: December 2018 - Volume 32 - Issue 12 - p 3373-3382. doi: 10.1519/JSC.0000000000002904Va 
  1. Vargas S, Romance R, Petro JL, et al. Efficacy of ketogenic diet on body composition during resistance training in trained men: a randomized controlled trial. J Int Soc Sports Nutr. 2018;15(1):31. Published 2018 Jul 9. doi:10.1186/s12970-018-0236-9 
  1. Miriam E. Clegg (2010) Medium-chain triglycerides are advantageous in promoting weight loss although not beneficial to exercise performance, International Journal of Food Sciences and Nutrition, 61:7, 653-679, DOI: 10.3109/09637481003702114 
  1. LaBarrie J, St-Onge MP. A coconut oil-rich meal does not enhance thermogenesis compared to corn oil in a randomized trial in obese adolescents. Insights Nutr Metab. 2017;1(1):30-36
  1. Farhat, G., Drummond, S., and Al‐Dujaili, E. A. S. (2017) Polyphenols and Their Role in Obesity Management: A Systematic Review of Randomized Clinical Trials. Phytother. Res., 31: 1005-1018. doi: 10.1002/ptr.5830.
  1. Sugita, J., Yoneshiro, T., Hatano, T., Aita, S., Ikemoto, T., Uchiwa, H., Saito, M. (2013). Grains of paradise (Aframomum melegueta) extract activates brown adipose tissue and increases whole-body energy expenditure in men. British Journal of Nutrition, 110(4), 733-738. doi:10.1017/S0007114512005715
  1. Jun Sugita, Takeshi Yoneshiro, Yuuki Sugishima, Takeshi Ikemoto, Hideyo Uchiwa, Isao Suzuki, Masayuki Saito, Daily Ingestion of Grains of Paradise (Aframomum melegueta) Extract Increases Whole-Body Energy Expenditure and Decreases Visceral Fat in Humans, Journal of Nutritional Science and Vitaminology, 2014, Volume 60, Issue 1, Pages 22-27, Released April 24, 2014, 
  1. Ilic NM, Dey M, Poulev AA, Logendra S, Kuhn PE, Raskin I. Anti-inflammatory activity of grains of paradise (Aframomum melegueta Schum) extract. J Agric Food Chem. 2014;62(43):10452-7. 
  1. Hattori, H., Yamauchi, K., Onwona‐Agyeman, S. and Mitsunaga, T. (2018), Identification of vanilloid compounds in grains of paradise and their effects on sympathetic nerve activity. J. Sci. Food Agric., 98: 4742-4748. doi:10.1002/jsfa.9009 
  1. The application of medium-chain fatty acids: edible oil with a suppressing effect on body fat accumulation.Hiroyuki Takeuchi, Seiji Sekine, Keiichi Kojima, Toshiaki Aoyama. Asia Pac J Clin Nutr. 2008; 17 (Suppl 1): 320–323. 
  1. Cliff J. d C. Harvey, Grant M. Schofield, Micalla Williden, and Joseph A. McQuillan. “The Effect of Medium Chain Triglycerides on Time to Nutritional Ketosis and Symptoms of Keto-Induction in Healthy Adults: A Randomised Controlled Clinical Trial,” Journal of Nutrition and Metabolism, vol. 2018, Article ID 2630565, 9 pages, 2018. https://doi.org/10.1155/2018/2630565. 
  1. King, DG, Walker, M, Campbell, MD, et al. (2018) A small dose of whey protein co-ingested with mixed-macronutrient breakfast and lunch meals improves postprandial glycemia and suppresses appetite in men with type 2 diabetes: a randomized controlled trial. Am J Clin Nutr 107, 550-557.
  1. Waldman, H. S., Basham, S. A., Price, F. G., Smith, J. W., Chander, H., Knight, A. C., McAllister, M. J. (2018). Exogenous ketone salts do not improve cognitive responses after a high-intensity exercise protocol in healthy college-aged males. Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition et Metabolisme, 43(7), 711-717. https://doi.org/10.1139/apnm-2017-0724
  1. Stubbs, B. J., Cox, P. J., Evans, R. D., Santer, P., Miller, J. J., Faull, O. K., Clarke, K. (2017). On the Metabolism of Exogenous Ketones in Humans. Frontiers in Physiology. Retrieved from https://www.frontiersin.org/article/10.3389/fphys.2017.00848
  1. Reid IR, Gamble GD, Bolland MJ. Circulating calcium concentrations, vascular disease and mortality: a systematic review. J Intern Med. 2016;279(6):524
  1. Wang L, Manson JE, Sesso HD. Calcium intake and risk of cardiovascular disease: a review of prospective studies and randomized clinical trials. Am J Cardiovasc Drugs. 2012;12(2):105-116. doi:10.2165/11595400-000000000-00000 
  1. The Thermo Heat® Weight Loss Revolution, by Michael J. Rudolph, Ph.D., including the foreword by Daniel L. Friedman, MD and Eugene B Friedman, MD.
  1. THE THERMO HEAT® LOW-CARB MEDITERRANEAN DIET: WHY IT’S THE HEALTHIEST & BEST DIET FOR 2019 by Steve Blechman, https://advancedmolecularlabs.com/blogs/news/the-thermo-heat%C2%AE-low-carb-mediterranean-diet-why-it-s-the-healthiest-best-diet-for-2019